Sulfated and sialylated <i>N</i>-glycans in the echinoderm <i>Holothuria atra</i> reflect its marine habitat and phylogeny.

ABSTRACT: Among the earliest deuterostomes, the echinoderms are an evolutionary important group of ancient marine animals. Within this phylum, the holothuroids (sea cucumbers) are known to produce a wide range of glycoconjugate biopolymers with apparent benefits to health; therefore, they are of economic and culinary interest throughout the world. Other than their highly modified glycosaminoglycans (e.g. fucosylated chondroitin sulfate and fucoidan), nothing is known about their protein-linked glycosylation. Here we used multistep N-glycan fractionation to efficiently separate anionic and neutral N-glycans before analyzing the N-glycans of the black sea cucumber (Holothuria atra) by MS in combination with enzymatic and chemical treatments. These analyses showed the

SUBMITTER: Vanbeselaere J 

PROVIDER: S-EPMC7062191 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature