Proteome Analysis of Isolated Podocytes Reveals Stress Responses in Glomerular Sclerosis.

ABSTRACT:

Background

Understanding podocyte-specific responses to injury at a systems level is difficult because injury leads to podocyte loss or an increase of extracellular matrix, altering glomerular cellular composition. Finding a window into early podocyte injury might help identify molecular pathways involved in the podocyte stress response.

Methods

We developed an approach to apply proteome analysis to very small samples of purified podocyte fractions. To examine podocytes in early disease states in FSGS mouse models, we used podocyte fractions isolated from individual mice after chemical induction of glomerular disease (with Doxorubicin or LPS). We also applied single-glomerular proteome analysis to tissue from patients with FSGS.

Results

Transcriptome and proteome anal

SUBMITTER: Koehler S 

PROVIDER: S-EPMC7062218 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature