Prostaglandin E₂ Induces miR675-5p to Promote Colorectal Tumor Metastasis via Modulation of p53 Expression.

ABSTRACT:

Background & aims

Prostaglandin E₂ (PGE₂) promotes colorectal tumor formation and progression by unknown mechanisms. We sought to identify microRNAs (miRNAs) that might mediate the effects of PGE₂ on colorectal cancer (CRC) development.

Methods

We incubated LS174T colorectal cancer cells with PGE₂ or without (control) and used miRNA-sequencing technology to compare expression patterns of miRNAs. We knocked down levels of specific miRNAs or proteins in cells using small interfering RNAs or genome editing. Cells were analyzed by immunoblot, quantitative polymerase chain reaction, chromosome immunoprecipitation, cell invasion, and luciferase reporter assays; we measured gene expression, binding activity, cell migration and invasion, a

SUBMITTER: Cen B 

PROVIDER: S-EPMC7062589 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature