Ontology highlight
ABSTRACT:
SUBMITTER: McCarty NS
PROVIDER: S-EPMC7062760 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
Nature communications 20200309 1
Multiplexed CRISPR technologies, in which numerous gRNAs or Cas enzymes are expressed at once, have facilitated powerful biological engineering applications, vastly enhancing the scope and efficiencies of genetic editing and transcriptional regulation. In this review, we discuss multiplexed CRISPR technologies and describe methods for the assembly, expression and processing of synthetic guide RNA arrays in vivo. Applications that benefit from multiplexed CRISPR technologies, including cellular r ...[more]