New role of the antidepressant imipramine as a Fascin1 inhibitor in colorectal cancer cells.

ABSTRACT: Serrated adenocarcinoma (SAC) is more invasive, has worse outcomes than conventional colorectal carcinoma (CRC), and is characterized by frequent resistance to anti-epidermal growth factor receptor (EGFR) and overexpression of fascin1, a key protein in actin bundling that plays a causative role in tumor invasion and is overexpressed in different cancer types with poor prognosis. In silico screening of 9591 compounds, including 2037 approved by the Food and Drug Administration (FDA), was performed, and selected compounds were analyzed for their fascin1 binding affinity by differential scanning fluorescence. The results were compared with migrastatin as a typical fascin1 inhibitor. In silico screening and differential scanning fluorescence yielded the FDA-approved antidepressant imipramine a

SUBMITTER: Alburquerque-Gonzalez B 

PROVIDER: S-EPMC7062870 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature