A novel and more efficient biosynthesis approach for human insulin production in Escherichia coli (E. coli).

ABSTRACT: Insulin has captured researchers' attention worldwide. There is a rapid global rise in the number of diabetic patients, which increases the demand for insulin. Current methods of insulin production are expensive and time-consuming. A PCR-based strategy was employed for the cloning and verification of human insulin. The human insulin protein was then overexpressed in E. coli on a laboratory scale. Thereafter, optimisation of human insulin expression was conducted. The yield of human insulin produced was approximately 520.92 (mg/L), located in the intracellular fraction. Human insulin was detected using the MALDI-TOF-MS and LC-MS methods. The crude biosynthesised protein sequence was verified using protein sequencing, which had a 100% similarity to the human insulin sequence. The biological

SUBMITTER: Govender K 

PROVIDER: S-EPMC7062966 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature