Physical and immunological barrier of human primary nasal epithelial cells from non-allergic and allergic donors.

ABSTRACT: The epithelial cell-derived cytokine milieu has been discussed as a "master switch" in the development of allergic disease. To understand the role of innate immune response in nasal epithelial cells during allergic inflammation, we created and established a fast and minimally invasive method to isolate and culture human nasal epithelial cells from clinically and immunologically well characterized patients. Human nasal epithelial cells from non-atopic volunteers and from allergic rhinitis patients were compared in respect to their growth, barrier integrity, pattern recognition, receptor expression, and immune responses to allergens and an array of pathogen-associated molecular patterns and inflammasome activators. Cells from nasal scrapings were clearly identified as nasal epithelial cells

SUBMITTER: Bergougnan C 

PROVIDER: S-EPMC7063333 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature