Intercellular Bridge Mediates Ca²⁺ Signals between Micropatterned Cells via IP₃ and Ca²⁺ Diffusion.

ABSTRACT: Intercellular bridges are plasma continuities formed at the end of the cytokinesis process that facilitate intercellular mass transport between the two daughter cells. However, it remains largely unknown how the intercellular bridge mediates Ca²⁺ communication between postmitotic cells. In this work, we utilize BV-2 microglial cells planted on dumbbell-shaped micropatterned assemblies to resolve spatiotemporal characteristics of Ca²⁺ signal transfer over the intercellular bridges. With the use of such micropatterns, considerably longer and more regular intercellular bridges can be obtained than in conventional cell cultures. The initial Ca²⁺ signal is evoked by mechanical stimulation of one of the daughter cells. A considerable time delay is observed betwee

SUBMITTER: Xing F 

PROVIDER: S-EPMC7063411 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature