Shear-Stress Sensitive Inwardly-Rectifying K⁺ Channels Regulate Developmental Retinal Angiogenesis by Vessel Regression.

ABSTRACT:

Background/aims

Shear stress plays major roles in developmental angiogenesis, particularly in blood vessel remodeling and maturation but little is known about the shear stress sensors involved in this process. Our recent study identified endothelial Kir2.1 channels as major contributors to flow-induced vasodilation, a hallmark of the endothelial flow response. The goal of this study is to establish the role of Kir2.1 in the regulation of retinal angiogenesis.

Methods

The retina of newly born Kir2.1^+/- mice were used to investigate the sprouting angiogenesis and remodeling of newly formed branched vessels. The structure, blood density and mural cell coverage have been evaluated by immunohistochemistry of the whole-mount retina. Endothelial cell alignment was assess

SUBMITTER: Boriushkin E 

PROVIDER: S-EPMC7063968 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature