ELF1-mediated LUCAT1 promotes choroidal melanoma by modulating RBX1 expression.

ABSTRACT: Long noncoding RNAs (lncRNAs) are essential regulators of gene expression and biological behaviors. However, the contribution of lncRNA LUCAT1 to choroidal melanoma (CM) remains unexplored. Here, we examined the expression of LUCAT1 in CM cells by qRT-PCR and investigated its biological effects by cell counting kit-8, EdU, TUNEL, transwell assays, and Western blot. Bioinformatics tools were applied to find RNA candidates for further study. Moreover, mechanistic experiments including RNA immunoprecipitation assay, pull-down assay, and luciferase reporter assay confirmed the relation or interaction among the indicated molecules. Here, we reported ELF1 as the transcription activator of LUCAT1. Functionally, elevated expression of LUCAT1 positively regulated CM cell proliferation, metastasis,

SUBMITTER: Wang L 

PROVIDER: S-EPMC7064025 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature