Parallel Genomics Uncover Novel Enterococcal-Bacteriophage Interactions.

ABSTRACT: Bacteriophages (phages) have been proposed as alternative therapeutics for the treatment of multidrug-resistant bacterial infections. However, there are major gaps in our understanding of the molecular events in bacterial cells that control how bacteria respond to phage predation. Using the model organism Enterococcus faecalis, we used two distinct genomic approaches, namely, transposon library screening and RNA sequencing, to investigate the interaction of E. faecalis with a virulent phage. We discovered that a transcription factor encoding a LytR family response regulator controls the expression of enterococcal polysaccharide antigen (epa) genes that are involved in phage infection and bacterial fitness. In addition, we discovered that DNA mismatch repair mutants rap

SUBMITTER: Chatterjee A 

PROVIDER: S-EPMC7064774 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature