Proteomics of human glomerulonephritis by laser microdissection and liquid chromatography-tandem mass spectrometry.

ABSTRACT:

Aim

Laser microdissection (LMD) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) enable clinicians to analyse proteins from tissue sections. In nephrology, these methods are used to diagnose diseases of abnormal protein deposition, such as amyloidosis, but they are seldom applied to the diagnosis and pathophysiological understanding of human glomerular diseases.

Methods

Renal biopsy specimens were obtained from five patients with IgA nephropathy (IgAN), five patients with membranous nephropathy (MN) and five kidney transplant donors (as controls). From 10-μm-thick sections of formalin-fixed, paraffin-embedded specimens, 0.3-mm² samples of glomerular tissue were subjected to LMD. The samples were analysed by LC-MS/MS and investigated clinically and his

SUBMITTER: Kawata N 

PROVIDER: S-EPMC7064884 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature