Effects of thyroid hormone on mitochondria and metabolism of human preimplantation embryos.

ABSTRACT: Thyroid hormones are regarded as the major controllers of metabolic rate and oxygen consumption in mammals. Although it has been demonstrated that thyroid hormone supplementation improves bovine embryo development in vitro, the cellular mechanisms underlying these effects are so far unknown. In this study, we investigated the role of thyroid hormone in development of human preimplantation embryos. Embryos were cultured in the presence or absence of 10^-7  M triiodothyronine (T3) till blastocyst stage. Inner cell mass (ICM) and trophectoderm (TE) were separated mechanically and subjected to RNAseq or quantification of mitochondrial DNA copy number. Analyses were performed using DESeq (v1.16.0 on R v3.1.3), MeV4.9 and MitoMiner 4.0^{v2018 JUN} platforms. We found that the e

SUBMITTER: Noli L 

PROVIDER: S-EPMC7064942 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature