Tumour-cell-derived complement components C1r and C1s promote growth of cutaneous squamous cell carcinoma.

ABSTRACT:

Background

The incidence of epidermal keratinocyte-derived cutaneous squamous cell carcinoma (cSCC) is increasing worldwide.

Objectives

To study the role of the complement classical pathway components C1q, C1r and C1s in the progression of cSCC.

Methods

The mRNA levels of C1Q subunits and C1R and C1S in cSCC cell lines, normal human epidermal keratinocytes, cSCC tumours in vivo and normal skin were analysed with quantitative real-time polymerase chain reaction. The production of C1r and C1s was determined with Western blotting. The expression of C1r and C1s in tissue samples in vivo was analysed with immunohistochemistry and further investigated in human cSCC xenografts by knocking down C1r and C1s.

Results

Significantly elevated C1R and C1S mRNA levels and pro

SUBMITTER: Riihila P 

PROVIDER: S-EPMC7065064 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature