Synergy at work: linking the metabolism of two lactic acid bacteria to achieve superior production of 2-butanol.

ABSTRACT:

Background

The secondary alcohol 2-butanol has many important applications, e.g., as a solvent. Industrially, it is usually made by sulfuric acid-catalyzed hydration of butenes. Microbial production of 2-butanol has also been attempted, however, with little success as witnessed by the low titers and yields reported. Two important reasons for this, are the growth-hampering effect of 2-butanol on microorganisms, and challenges associated with one of the key enzymes involved in its production, namely diol dehydratase.

Results

We attempt to link the metabolism of an engineered Lactococcus lactis strain, which possesses all enzyme activities required for fermentative production of 2-butanol from glucose, except for diol dehydratase, which acts on meso-2,3-butanediol

SUBMITTER: Mar MJ 

PROVIDER: S-EPMC7065357 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature