Multi-ATOM: Ultrahigh-throughput single-cell quantitative phase imaging with subcellular resolution.

ABSTRACT: A growing body of evidence has substantiated the significance of quantitative phase imaging (QPI) in enabling cost-effective and label-free cellular assays, which provides useful insights into understanding the biophysical properties of cells and their roles in cellular functions. However, available QPI modalities are limited by the loss of imaging resolution at high throughput and thus run short of sufficient statistical power at the single-cell precision to define cell identities in a large and heterogeneous population of cells-hindering their utility in mainstream biomedicine and biology. Here we present a new QPI modality, coined multiplexed asymmetric-detection time-stretch optical microscopy (multi-ATOM) that captures and processes quantitative label-free single-cell images at ultrah

SUBMITTER: Lee KCM 

PROVIDER: S-EPMC7065649 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature