Reovirus uses macropinocytosis-mediated entry and fast axonal transport to infect neurons.

ABSTRACT: Several barriers protect the central nervous system (CNS) from pathogen invasion. Yet viral infections of the CNS are common and often debilitating. Understanding how neurotropic viruses co-opt host machinery to overcome challenges to neuronal entry and transmission is important to combat these infections. Neurotropic reovirus disseminates through neural routes and invades the CNS to cause lethal encephalitis in newborn animals. To define mechanisms of reovirus neuronal entry and directional transport, we used primary neuron cultures, which reproduce in vivo infection patterns displayed by different reovirus serotypes. Treatment of neurons with small-molecule inhibitors of different endocytic uptake pathways allowed us to discover that the cellular machinery mediating macropinocytosis is r

SUBMITTER: Aravamudhan P 

PROVIDER: S-EPMC7065821 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature