N⁶-Methyladenosine METTL3 Modulates the Proliferation and Apoptosis of Lens Epithelial Cells in Diabetic Cataract.

ABSTRACT: N⁶-methyladenosine (m⁶A) is the most prevalent eukaryotic messenger RNA modification. Diabetic cataract (DC) is caused by high glucose (HG) in diabetes mellitus. However, the regulatory mechanism of m⁶A in the DC pathogenesis is poorly understood. In present research, we performed the m⁶A-RNA immunoprecipitation sequencing (MeRIP-Seq) analysis and detected the m⁶A modification profile in the HG- or normal glucose (NG)-induced human lens epithelial cells (HLECs). Results revealed that methyltransferase-like 3 (METTL3) was upregulated in the DC tissue specimens and HG-induced HLECs. Besides, total m⁶A modification level was higher in the HG-induced HLECs. Functionally, METTL3 knockdown promoted the proliferation and repressed

SUBMITTER: Yang J 

PROVIDER: S-EPMC7066033 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature