UBB pseudogene 4 encodes functional ubiquitin variants.

ABSTRACT: Pseudogenes are mutated copies of protein-coding genes that cannot be translated into proteins, but a small subset of pseudogenes has been detected at the protein level. Although ubiquitin pseudogenes represent one of the most abundant pseudogene families in many organisms, little is known about their expression and signaling potential. By re-analyzing public RNA-sequencing and proteomics datasets, we here provide evidence for the expression of several ubiquitin pseudogenes including UBB pseudogene 4 (UBBP4), which encodes Ub^KEKS (Q2K, K33E, Q49K, N60S). The functional consequences of Ub^KEKS conjugation appear to differ from canonical ubiquitylation. Quantitative proteomics shows that Ub^KEKS modifies specific proteins including lamins. Knockout of UBBP4 res

SUBMITTER: Dubois ML 

PROVIDER: S-EPMC7066184 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature