Quantitative imaging of sleep behavior in Caenorhabditis elegans and larval Drosophila melanogaster.

ABSTRACT: Sleep is nearly universal among animals, yet remains poorly understood. Recent work has leveraged simple model organisms, such as Caenorhabditis elegans and Drosophila melanogaster larvae, to investigate the genetic and neural bases of sleep. However, manual methods of recording sleep behavior in these systems are labor intensive and low in throughput. To address these limitations, we developed methods for quantitative imaging of individual animals cultivated in custom microfabricated multiwell substrates, and used them to elucidate molecular mechanisms underlying sleep. Here, we describe the steps necessary to design, produce, and image these plates, as well as analyze the resulting behavioral data. We also describe approaches for experimentally manipulating sleep. Following these procedu

SUBMITTER: Churgin MA 

PROVIDER: S-EPMC7066577 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature