RapidAIM: a culture- and metaproteomics-based Rapid Assay of Individual Microbiome responses to drugs.

ABSTRACT:

Background

Human-targeted drugs may exert off-target effects or can be repurposed to modulate the gut microbiota. However, our understanding of such effects is limited due to a lack of rapid and scalable assay to comprehensively assess microbiome responses to drugs. Drugs and other compounds can drastically change the overall abundance, taxonomic composition, and functions of a gut microbiome.

Results

Here, we developed an approach to screen compounds against individual microbiomes in vitro, using metaproteomics to both measure absolute bacterial abundances and to functionally profile the microbiome. Our approach was evaluated by testing 43 compounds (including 4 antibiotics) against 5 individual microbiomes. The method generated technically highly reproducible readouts, inc

SUBMITTER: Li L 

PROVIDER: S-EPMC7066843 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature