Blasticidin S Deaminase: A New Efficient Selectable Marker for Chlamydomonas reinhardtii.

ABSTRACT: Chlamydomonas reinhardtii is a model unicellular organism for basic or biotechnological research, such as the production of high-value molecules or biofuels thanks to its photosynthetic ability. To enable rapid construction and optimization of multiple designs and strains, our team and collaborators have developed a versatile Chlamydomonas Modular Cloning toolkit comprising 119 biobricks. Having the ability to use a wide range of selectable markers is an important benefit for forward and reverse genetics in Chlamydomonas. We report here the development of a new selectable marker based on the resistance to the antibiotic blasticidin S, using the Bacillus cereus blasticidin S deaminase (BSR) gene. The optimal concentration of blasticidin S for effective selection was det

SUBMITTER: de Carpentier F 

PROVIDER: S-EPMC7066984 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature