A Singular System with Precise Dosing and Spatiotemporal Control of CRISPR-Cas9.

ABSTRACT: Several genome engineering applications of CRISPR-Cas9, an RNA-guided DNA endonuclease, require precision control of Cas9 activity over dosage, timing, and targeted site in an organism. While some control of Cas9 activity over dose and time have been achieved using small molecules, and spatial control using light, no singular system with control over all the three attributes exists. Furthermore, the reported small-molecule systems lack wide dynamic range, have background activity in the absence of the small-molecule controller, and are not biologically inert, while the optogenetic systems require prolonged exposure to high-intensity light. We previously reported a small-molecule-controlled Cas9 system with some dosage and temporal control. By photocaging this Cas9 activator to render it bi

SUBMITTER: Manna D 

PROVIDER: S-EPMC7067309 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature