Transient pentameric IgM fulfill biological function-Effect of expression host and transfection on IgM properties.

ABSTRACT: Recombinant production of IgM antibodies poses a special challenge due to the complex structure of the proteins and their not yet fully elucidated interactions with the immune effector proteins, especially the complement system. In this study, we present transient expression of IgM antibodies (IgM617, IgM012 and IgM012_GL) in HEK cells and compared it to the well-established stable expression system in CHO cells. The presented workflow investigates quality attributes including productivity, polymer distribution, glycosylation, antibody structure and activation of the classical complement pathway. The HEK293E transient expression system is able to generate comparable amounts and polymer distribution as IgM stably produced in CHO. Although the glycan profile generated by HEK293E cells contai

SUBMITTER: Hennicke J 

PROVIDER: S-EPMC7067452 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature