Alteration of Cellular Reduction Potential Will Change ⁶⁴Cu-ATSM Signal With or Without Hypoxia.

ABSTRACT: Therapies targeting reductive/oxidative (redox) metabolism hold potential in cancers resistant to chemotherapy and radiation. A redox imaging marker would help identify cancers susceptible to redox-directed therapies. Copper(II)-diacetyl-bis(4-methylthiosemicarbazonato) (Cu-ATSM) is a PET tracer developed for hypoxia imaging that could potentially be used for this purpose. We aimed to demonstrate that Cu-ATSM signal is dependent on cellular redox state, irrespective of hypoxia. Methods: We investigated the relationship between ⁶⁴Cu-ATSM signal and redox state in human cervical and colon cancer cells. We altered redox state using drug strategies and single-gene mutations in isocitrate dehydrogenases (IDH1/2). Concentrations of reducing molecules were determined by spectrop

SUBMITTER: Floberg JM 

PROVIDER: S-EPMC7067520 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature