Quantification of [¹¹C]PBR28 data after systemic lipopolysaccharide challenge.

ABSTRACT:

Background

Lipopolysaccharide (LPS) is a classic immune stimulus. LPS combined with positron emission tomography (PET) 18 kDa translocator protein (TSPO) brain imaging provides a robust human laboratory model to study neuroimmune signaling. To evaluate optimal analysis of these data, this work compared the sensitivity of six quantification approaches.

Methods

[¹¹C]PBR28 data from healthy volunteers (N = 8) were collected before and 3 h after LPS challenge (1.0 ng/kg IV). Quantification approaches included total volume of distribution estimated with two tissue, and two tissue plus irreversible uptake in whole blood, compartment models (2TCM and 2TCM-1k, respectively) and multilinear analysis-1 (MA-1); binding potential estimated with simultaneous estimation (SIME);

SUBMITTER: Woodcock EA 

PROVIDER: S-EPMC7067964 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature