Cigarette smoke and electronic cigarettes differentially activate bronchial epithelial cells.

ABSTRACT:

Background

The use of electronic cigarettes (ECIGs) is increasing, but the impact of ECIG-vapor on cellular processes like inflammation or host defense are less understood. The aim of the present study was to compare the acute effects of traditional cigarettes (TCIGs) and ECIG-exposure on host defense, inflammation, and cellular activation of cell lines and primary differentiated human airway epithelial cells (pHBE).

Methods

We exposed pHBEs and several cell lines to TCIG-smoke or ECIG-vapor. Epithelial host defense and barrier integrity were determined. The transcriptome of airway epithelial cells was compared by gene expression array analysis. Gene interaction networks were constructed and differential gene expression over all groups analyzed. The expression of several can

SUBMITTER: Herr C 

PROVIDER: S-EPMC7068890 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature