Machine Learning with Optical Phase Signatures for Phenotypic Profiling of Cell Lines.

ABSTRACT: Robust and reproducible profiling of cell lines is essential for phenotypic screening assays. The goals of this study were to determine robust and reproducible optical phase signatures of cell lines for classification with machine learning and to correlate optical phase parameters to motile behavior. Digital holographic microscopy (DHM) reconstructed phase maps of cells from two pairs of cancer and non-cancer cell lines. Seventeen image parameters were extracted from each cell's phase map, used for linear support vector machine learning, and correlated to scratch wound closure and Boyden chamber chemotaxis. The classification accuracy was between 90% and 100% for the six pairwise cell line comparisons. Several phase parameters correlated with wound closure rate and chemotaxis across the fo

SUBMITTER: Lam VK 

PROVIDER: S-EPMC7068951 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature