Rapamycin promotes endothelial-mesenchymal transition during stress-induced premature senescence through the activation of autophagy.

ABSTRACT:

Background

Rapamycin is known to be effective in suppressing senescence and the senescence-associated secretory phenotype (SASP). Therefore, it is highly expected to represent an anti-aging drug. Its anti-aging effect has been demonstrated at the mouse individual level. However, there are not many clinical findings with respect to its activity in humans. Here, we aimed to clarify the effect of rapamycin on human endothelial cells (ECs) as an in vitro model of human blood vessels.

Methods

Over the course of oxidative stress-induced senescence using hydrogen peroxide, we examined the effect of rapamycin on human coronary artery ECs (HCAECs). Senescence was evaluated by detecting senescence-associated β-galactosidase (SA-β-Gal) activity and the real-time PCR analysis of p16

SUBMITTER: Sasaki N 

PROVIDER: S-EPMC7069020 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature