ROCK inhibitor increases proacinar cells in adult salivary gland organoids.

ABSTRACT: Salisphere-derived adult epithelial cells have been used to improve saliva production of irradiated mouse salivary glands. Importantly, optimization of the cellular composition of salispheres could improve their regenerative capabilities. The Rho Kinase (ROCK) inhibitor, Y27632, has been used to increase the proliferation and reduce apoptosis of progenitor cells grown in vitro. In this study, we investigated whether Y27632 could be used to improve expansion of adult submandibular salivary epithelial progenitor cells or to affect their differentiation potential in different media contexts. Application of Y27632 in medium used previously to grow salispheres promoted expansion of Kit⁺ and Mist1⁺ cells, while in simple serum-containing medium Y27632 increased the number o

SUBMITTER: Koslow M 

PROVIDER: S-EPMC7069099 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature