The EUCAST rapid disc diffusion method for antimicrobial susceptibility testing directly from positive blood culture bottles.

ABSTRACT:

Objectives

With increasing antimicrobial resistance, rapid antimicrobial susceptibility testing (RAST) becomes important, especially in patients with bloodstream infections. EUCAST decided to develop a standardized rapid method, based on EUCAST disc diffusion, to offer susceptibility reports within 4-8 h of a positive blood culture (BC).

Methods

BC bottles were spiked with clinical isolates (n = 332) of the seven most relevant sepsis pathogens with a variety of resistance mechanisms. RAST was performed directly from the bottle and zones read after 4, 6 and 8 h. Several variables were investigated, including the effect of using different BC bottles and of a 0-18 h delay between a positive signal and the performance of RAST.

Results

For five species, most inhibition zon

SUBMITTER: Jonasson E 

PROVIDER: S-EPMC7069491 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature