Construction of a self-directed replication system for label-free and real-time sensing of repair glycosylases with zero background.

ABSTRACT: Genomic DNA damage and repair are involved in multiple fundamental biological processes, including metabolism, disease, and aging. Inspired by the natural repair mechanism in vivo, we demonstrate for the first time the construction of a self-directed replication system for label-free and real-time sensing of repair glycosylases with zero background. The presence of DNA glycosylase can catalyze the excision repair of the damaged base, successively autostarting the self-directed replication through recycling polymerization extension and strand-displacement DNA synthesis for the generation of exponentially amplified dsDNAs. The resultant dsDNA products can be label-free and real-time monitored with SYBR Green I as the fluorescent indicator. Owing to the high efficiency of self-directed

SUBMITTER: Wang LJ 

PROVIDER: S-EPMC7069502 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature