Rapid diagnosis of periodontitis, a feasibility study using MALDI-TOF mass spectrometry.

ABSTRACT: AIM:The aim of the present study was to assess the feasibility and diagnostic contribution of protein profiling using MALDI-TOF mass spectrometry applied to saliva, gingival crevicular fluid (GCF) and dental plaque from periodontitis and healthy subjects. We hypothesized that rapid routine and blinded MALDI-TOF analysis could accurately classify these three types of samples according to periodontal state. MATERIALS AND METHODS:Unstimulated saliva, GCF and dental plaque, collected from periodontitis subjects and healthy controls, were analyzed by MALDI-TOF MS. Based on the differentially expressed peaks between the two groups, diagnostic decision trees were built for each sample. RESULTS:Among 141 patients (67 periodontitis and 74 healthy controls), the decision trees diagnosed periodontiti

SUBMITTER: Antezack A 

PROVIDER: S-EPMC7069628 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature