Cell-type-specific role of CHK2 in mediating DNA damage-induced G2 cell cycle arrest.

ABSTRACT: Cancer is a life-threatening disease that affects one in three people. Although most cases are sporadic, cancer risk can be increased by genetic factors. It remains unknown why certain genes predispose for specific forms of cancer only, such as checkpoint protein 2 (CHK2), in which gene mutations convey up to twofold higher risk for breast cancer but do not increase lung cancer risk. We have investigated the role of CHK2 and the related kinase checkpoint protein 1 (CHK1) in cell cycle regulation in primary breast and lung primary epithelial cells. At the molecular level, CHK1 activity was higher in lung cells, whereas CHK2 was more active in breast cells. Inhibition of CHK1 profoundly disrupted the cell cycle profile in both lung and breast cells, whereas breast cells were more sensitive t

SUBMITTER: van Jaarsveld MTM 

PROVIDER: S-EPMC7070093 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature