Metagenomic Insights into Effects of Thiamine Supplementation on Carbohydrate-Active Enzymes' Profile in Dairy Cows Fed High-Concentrate Diets.

ABSTRACT: As the co-enzyme of pyruvate formate-lyase under ruminal anaerobic condition, thiamine plays a critical role in carbohydrate metabolism in dairy cows. The objective of this study was to investigate the impacts of thiamine supplementation on ruminal carbohydrate-active enzymes. Twelve Holstein dairy cows were randomly assigned into three dietary treatments: control diet (CON; 20% starch, dry matter (DM) basis), high-concentrate diet (HC; 33.2% starch, DM basis) and a high-concentrate diet supplemented with 180 mg thiamine/kg DM (HCT; 33.2% starch, DM basis). Dry matter intake and milk production were recorded for 21 days. Rumen fluid samples were collected, and ruminal pH and volatile fatty acids (VFAs) were measured. The metagenome sequencing technique was used to detect the genes in rumin

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC7070242 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature