An MRM-Based Multiplexed Quantification Assay for Human Adipokines and Apolipoproteins.

ABSTRACT: Adipokines and apolipoproteins are key regulators and potential biomarkers in obesity and associated diseases and their quantitative assessment is crucial for functional analyses to understand disease mechanisms. Compared to routinely used ELISAs, multiple reaction monitoring (MRM)-based mass spectrometry allows multiplexing and detection of proteins for which antibodies are not available. Thus, we established an MRM method to quantify 9 adipokines and 10 apolipoproteins in human serum. We optimized sample preparation by depleting the two most abundant serum proteins for improved detectability of low abundant proteins. Intra-day and inter-day imprecision were below 16.5%, demonstrating a high accuracy. In 50 serum samples from participants with either normal weight or obesity, we quantifie

SUBMITTER: Krieg L 

PROVIDER: S-EPMC7070386 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature