Detection of Salmonella Enterica in Egg Yolk by PCR on a Microfluidic Disc Device Using Immunomagnetic Beads.

ABSTRACT: Salmonella enterica is a pathogenic bacterium that causes foodborne illness. One of the vehicle foods of S.enterica are chicken eggs. Efficient collection of the bacterium is necessary to detect it specifically. We developed a method to detect S.enterica by PCR on a microfluidic disc device using a fluorescent probe. Salmonella enterica cells were isolated in the microchambers on the device, followed by thermal lysis and PCR targeting with the invA gene, a gene specific to S.enterica, were observed by measurement of the fluorescent signal that resulted from gene amplification. However, the developed method was unable to discriminate viable cells from dead cells. Consequently, in this study, magnetic beads modified with anti-Salmonella antibody were utilized to detect viable Salmonella cell

SUBMITTER: Kubo I 

PROVIDER: S-EPMC7070913 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature