Fenobody and RANbody-based sandwich enzyme-linked immunosorbent assay to detect Newcastle disease virus.

ABSTRACT:

Background

Traditional sandwich enzyme-linked immunosorbent assay (ELISA) using polyclonal and monoclonal antibodies as reagents presents several drawbacks, including limited amounts, difficulty in permanent storage, and required use of a secondary antibody. Nanobodies can be easily expressed with different systems and fused with several tags in their tertiary structure by recombinant technology, thus offering an effective detection method for diagnostic purposes. Recently, the fenobody (ferritin-fused nanobody) and RANbody (nanobody-fused reporter) have been designed and derived from the nanobody for developing the diagnostic immunoassays. However, there was no report about developing the sandwich ELISA using the fenobody and RANbody as pairing reagents.

Results

A platform

SUBMITTER: Ji P 

PROVIDER: S-EPMC7071587 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature