Improving L-serine formation by Escherichia coli by reduced uptake of produced L-serine.

ABSTRACT: BACKGROUND:Microbial de novo production of L-serine, which is widely used in a range of cosmetic and pharmaceutical products, has attracted increasing attention due to its environmentally friendly characteristics. Previous pioneering work mainly focused on L-serine anabolism; however, in this study, it was found that L-serine could be reimported through the L-serine uptake system, thus hampering L-serine production. RESULT:To address this challenge, engineering via deletion of four genes, namely, sdaC, cycA, sstT and tdcC, which have been reported to be involved in L-serine uptake in Escherichia coli, was first carried out in the L-serine producer E. coli ES. Additionally, the effects of these genes on L-serine uptake activity and L-serine production were investigated. The data revealed an

SUBMITTER: Wang C 

PROVIDER: S-EPMC7071685 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature