Umbilical cord-derived CD362⁺ mesenchymal stromal cells for E. coli pneumonia: impact of dose regimen, passage, cryopreservation, and antibiotic therapy.

ABSTRACT:

Background

Mesenchymal stromal cells (MSCs) demonstrate considerable promise for acute respiratory distress syndrome (ARDS) and sepsis. However, standard approaches to MSC isolation generate highly heterogeneous cell populations, while bone marrow (BM) constitutes a limited and difficult to access MSC source. Furthermore, a range of cell manufacturing considerations and clinical setting practicalities remain to be explored.

Methods

Adult male rats were subject to E. coli-induced pneumonia and administered CD362⁺ umbilical cord (UC)-hMSCs using a variety of cell production and clinical relevance considerations. In series 1, animals were instilled with E. coli and randomized to receive heterogeneous BM or UC-hMSCs or CD362⁺ UC-hMSCs. Subsequent series exa

SUBMITTER: Horie S 

PROVIDER: S-EPMC7071745 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature