The ATAD2/ANCCA homolog Yta7 cooperates with Scm3^HJURP to deposit Cse4^CENP-A at the centromere in yeast.

ABSTRACT: The AAA⁺ ATPase and bromodomain factor ATAD2/ANCCA is overexpressed in many types of cancer, but how it contributes to tumorigenesis is not understood. Here, we report that the Saccharomyces cerevisiae homolog Yta7^ATAD2 is a deposition factor for the centromeric histone H3 variant Cse4^CENP-A at the centromere in yeast. Yta7^ATAD2 regulates the levels of centromeric Cse4^CENP-A in that yta7∆ causes reduced Cse4^CENP-A deposition, whereas YTA7 overexpression causes increased Cse4^CENP-A deposition. Yta7^ATAD2 coimmunoprecipitates with Cse4^CENP-A and is associated with the centromere, arguing for a direct role of Yta7^ATAD2 in Cse4^CENP-A deposition. Furthermo

SUBMITTER: Shahnejat-Bushehri S 

PROVIDER: S-EPMC7071854 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature