Joint profiling of chromatin accessibility and CAR-T integration site analysis at population and single-cell levels.

ABSTRACT: Chimeric antigen receptor (CAR)-T immunotherapy has yielded impressive results in several B cell malignancies, establishing itself as a powerful means to redirect the natural properties of T lymphocytes. In this strategy, the T cell genome is modified by the integration of lentiviral vectors encoding CAR that direct tumor cell killing. However, this therapeutic approach is often limited by the extent of CAR-T cell expansion in vivo. A major outstanding question is whether or not CAR-T integration itself enhances the proliferative competence of individual T cells by rewiring their regulatory landscape. To address this question, it is critical to define the identity of an individual CAR-T cell and simultaneously chart where the CAR-T vector integrates into the genome. Here, we report the dev

SUBMITTER: Wang W 

PROVIDER: S-EPMC7071901 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature