¹H magnetic resonance spectroscopy of ²H-to-¹H exchange quantifies the dynamics of cellular metabolism in vivo.

ABSTRACT: Quantitative mapping of the in vivo dynamics of cellular metabolism via non-invasive imaging contributes to our understanding of the initiation and progression of diseases associated with dysregulated metabolic processes. Current methods for imaging cellular metabolism are limited by low sensitivities, costs or the use of specialized hardware. Here, we introduce a method that captures the turnover of cellular metabolites by quantifying signal reductions in proton magnetic resonance spectroscopy (MRS) resulting from the replacement of ¹H with ²H. The method, which we termed quantitative exchanged-label turnover MRS, only requires deuterium-labelled glucose and standard magnetic resonance imaging scanners, and with a single acquisition provides steady-state information

SUBMITTER: Rich LJ 

PROVIDER: S-EPMC7071956 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature