High levels of genetically intact HIV in HLA-DR+ memory T cells indicates their value for reservoir studies.

ABSTRACT:

Objective

The contribution of HLA-DR+ memory CD4 T cells to the HIV reservoir during prolonged antiretroviral therapy is unclear as these cells are commonly excluded when assessing for replication-competent HIV. To address this issue, we examined the distribution of genetically intact HIV DNA within HLA-DR- and HLA-DR+ memory CD4 T cells and the RNA transcriptional profile of these cells during antiretroviral therapy.

Design/methods

Full-length DNA sequencing was used to examine the HIV DNA landscape within HLA-DR+ and HLA-DR- memory CD4 T cells. RNA quantification and sequencing was used to interrogate the relationship between HLA-DR status and HIV RNA transcription.

Results

HLA-DR+ CD4 T cells contained a high frequency of genetically intact HIV genomes, contributin

SUBMITTER: Horsburgh BA 

PROVIDER: S-EPMC7071960 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature