Cell-Surface Proteomic Profiling in the Fly Brain Uncovers Wiring Regulators.

ABSTRACT: Molecular interactions at the cellular interface mediate organized assembly of single cells into tissues and, thus, govern the development and physiology of multicellular organisms. Here, we developed a cell-type-specific, spatiotemporally resolved approach to profile cell-surface proteomes in intact tissues. Quantitative profiling of cell-surface proteomes of Drosophila olfactory projection neurons (PNs) in pupae and adults revealed global downregulation of wiring molecules and upregulation of synaptic molecules in the transition from developing to mature PNs. A proteome-instructed in vivo screen identified 20 cell-surface molecules regulating neural circuit assembly, many of which belong to evolutionarily conserved protein families not previously linked to neural development. Genetic ana

SUBMITTER: Li J 

PROVIDER: S-EPMC7072036 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature