Nuclear Export of Cyclin B Mediated by the Nup62 Complex Is Required for Meiotic Initiation in Drosophila Males.

ABSTRACT:

Background

The central channel of the nuclear pore complex plays an important role in the selective transport of proteins between the nucleus and cytoplasm. Previous studies have demonstrated that the depletion of the Nup62 complex, constructing the nuclear pore channel in premeiotic Drosophila cells, resulted in the absence of meiotic cells. We attempted to understand the mechanism underlying the cell cycle arrest before meiosis.

Methods

We induced dsRNAs against the nucleoporin mRNAs using the Gal4/UAS system in Drosophila.

Results

The cell cycle of the Nup62-depleted cells was arrested before meiosis without CDK1 activation. The ectopic over-expression of CycB, but not constitutively active CDK1, resulted in partial rescue from the arrest. CycB contin

SUBMITTER: Okazaki R 

PROVIDER: S-EPMC7072204 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature