Excess TPX2 Interferes with Microtubule Disassembly and Nuclei Reformation at Mitotic Exit.

ABSTRACT: The microtubule-associated protein TPX2 is a key mitotic regulator that contributes through distinct pathways to spindle assembly. A well-characterised function of TPX2 is the activation, stabilisation and spindle localisation of the Aurora-A kinase. High levels of TPX2 are reported in tumours and the effects of its overexpression have been investigated in cancer cell lines, while little is known in non-transformed cells. Here we studied TPX2 overexpression in hTERT RPE-1 cells, using either the full length TPX2 or a truncated form unable to bind Aurora-A, to identify effects that are dependent-or independent-on its interaction with the kinase. We observe significant defects in mitotic spindle assembly and progression through mitosis that are more severe when overexpressed TPX2 is able to

SUBMITTER: Naso FD 

PROVIDER: S-EPMC7072206 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature