Generation and Characterization of a CRISPR/Cas9 -Induced 3-mst Deficient Zebrafish.

ABSTRACT: 3-mercaptopyruvate sulfurtransferase (3-MST) is an enzyme capable of synthesizing hydrogen sulfide (H₂S) and polysulfides. In spite of its ubiquitous presence in mammalian cells, very few studies have investigated its contribution to homeostasis and disease development, thus the role of 3-MST remains largely unexplored. Here, we present a clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated protein-9 (Cas9) induced 3-mst mutant zebrafish line, which will allow the study of 3-MST's role in several biological processes. The 3-mst zebrafish orthologue was identified using a bioinformatic approach and verified by its ability to produce H₂S in the presence of 3-mercaptopyruvate (3-MP). Its expression pattern was analyzed duri

SUBMITTER: Katsouda A 

PROVIDER: S-EPMC7072312 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature