Design and Characterization of an "All-in-One" Lentiviral Vector System Combining Constitutive Anti-G_D2 CAR Expression and Inducible Cytokines.

ABSTRACT: Genetically modified T cells expressing chimeric antigen receptors (CARs) so far have mostly failed in the treatment of solid tumors owing to a number of limitations, including an immunosuppressive tumor microenvironment and insufficient CAR T cell activation and persistence. Next-generation approaches using CAR T cells that secrete transgenic immunomodulatory cytokines upon CAR signaling, known as TRUCKs ("T cells redirected for universal cytokine-mediated killing"), are currently being explored. As TRUCKs were engineered by the transduction of T cells with two separate vectors, we developed a lentiviral modular "all-in-one" vector system that combines constitutive CAR expression and inducible nuclear factor of activated T cells (NFAT)-driven transgene expression for more efficient produc

SUBMITTER: Zimmermann K 

PROVIDER: S-EPMC7072617 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature