N⁶-Adenosine Methylation in RNA and a Reduced m₃G/TMG Level in Non-Coding RNAs Appear at Microirradiation-Induced DNA Lesions.

ABSTRACT: The DNA damage response is mediated by both DNA repair proteins and epigenetic markers. Here, we observe that N⁶-methyladenosine (m⁶A), a mark of the epitranscriptome, was common in RNAs accumulated at UV-damaged chromatin; however, inhibitors of RNA polymerases I and II did not affect the m⁶A RNA level at the irradiated genomic regions. After genome injury, m⁶A RNAs either diffused to the damaged chromatin or appeared at the lesions enzymatically. DNA damage did not change the levels of METTL3 and METTL14 methyltransferases. In a subset of irradiated cells, only the METTL16 enzyme, responsible for m⁶A in non-coding RNAs as well as for splicing regulation, was recruited to microirradiated sites. Importantly, the levels of the studied s

SUBMITTER: Svobodova Kovarikova A 

PROVIDER: S-EPMC7072662 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature